We are happy to share our optimized protocol for extracting high molecular weight bacterial DNA from field-collected fecal samples. This protocol has been designed to obtain fecal DNA suitable for long read metagenomic sequencing on the portable MinION instrument enabling field-sequencing of fecal samples. Try it on your own samples! Magdeleine A., Tilak M.-K., Teullet S. & Delsuc F. (2021). High molecular weight bacterial DNA extraction from field-collected fecal samples pre

We are happy to share our optimized protocol for sequencing long reads from mammalian roadkill tissues with the Oxford Nanopore Technology (ONT). This protocol was specifically developed to optimize DNA extraction and sequencing roadkill tissues containing necrotized cells and impurities that result in DNA degradation. First, we observed that DNA preservation was generally better in RNAlater than in 95% EtOH preserved tissues. Second, we found that physically removing necroti